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trademarks  (agena bioscience)


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    Structured Review

    agena bioscience trademarks
    Trademarks, supplied by agena bioscience, used in various techniques. Bioz Stars score: 99/100, based on 3312 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/spectrochip/trademarks/custom%40trademarks%4042618957
    Average 99 stars, based on 3312 article reviews
    trademarks - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: A MALDI-TOF mass spectrometry-based method for detection of copy number variations in BRCA1 and BRCA2 genes.
    Article Snippet: All the exons of BRCA1 and BRCA2 were examined in a total of four multiplex PCR amplification and extension reactions Frontiers in Molecular Biosciences frontiersin.org02 which were carried out using iPLEX Pro Reagents Kit (Agena Bioscience) according to the manufacturer’s instructions. .. Briefly, equal copies of gDNA and competitors were inputted into one tube and PCR amplification was performed as followings: 30°C for 10 min followed by 96°C for 2 min, then 5 cycles of 96°C 45 s, 65°C 30 s and 72°C 1 min, 40 cycles of 96°C 45 s, 60°C 30 s and 72°C 1 min, with a final step of 72°C 5 min. PCR products were then treated with shrimp alkaline phosphatase, followed by a single-base extension which was performed as followings: 97°C 45 s, 97°C 5 s plus 5 repetitions of 52°C 5 s and 80°C 5 s for 40 cycles, and then 72°C 3 min. After extension, the products were desalted and spotted onto a SpectroCHIP (Agena Bioscience) using the MassARRAY Chip Prep Module (Agena Bioscience). .. SpectroCHIPs were scanned using the MassARRAYTM Analyzer 4 (Agena Bioscience) and the spectra were processed using the TYPERTM (Agena Bioscience) software to get the peak intensity for each allele.

    Article Title: LIN28B Polymorphisms Confer a Higher Postoperative Recurrence Risk in Reproductive-Age Women with Endometrial Polyps
    Article Snippet: .. The PCR products were purified by resin and added into a SpectroCHIP (Agena Bioscience, Sequenom, San Diego, California, USA), then were analyzed by MALDI-TOF techniques. ..

    Amplification:

    Article Title: A MALDI-TOF mass spectrometry-based method for detection of copy number variations in BRCA1 and BRCA2 genes.
    Article Snippet: All the exons of BRCA1 and BRCA2 were examined in a total of four multiplex PCR amplification and extension reactions Frontiers in Molecular Biosciences frontiersin.org02 which were carried out using iPLEX Pro Reagents Kit (Agena Bioscience) according to the manufacturer’s instructions. .. Briefly, equal copies of gDNA and competitors were inputted into one tube and PCR amplification was performed as followings: 30°C for 10 min followed by 96°C for 2 min, then 5 cycles of 96°C 45 s, 65°C 30 s and 72°C 1 min, 40 cycles of 96°C 45 s, 60°C 30 s and 72°C 1 min, with a final step of 72°C 5 min. PCR products were then treated with shrimp alkaline phosphatase, followed by a single-base extension which was performed as followings: 97°C 45 s, 97°C 5 s plus 5 repetitions of 52°C 5 s and 80°C 5 s for 40 cycles, and then 72°C 3 min. After extension, the products were desalted and spotted onto a SpectroCHIP (Agena Bioscience) using the MassARRAY Chip Prep Module (Agena Bioscience). .. SpectroCHIPs were scanned using the MassARRAYTM Analyzer 4 (Agena Bioscience) and the spectra were processed using the TYPERTM (Agena Bioscience) software to get the peak intensity for each allele.

    Purification:

    Article Title: DNA methylation-mediated silencing of miR-204 is a potential prognostic marker for papillary thyroid carcinoma
    Article Snippet: RNase A cleavage (MassCleave Kit; Agena Bioscience, Inc.) was used for the reverse. .. The purified samples were spotted on a 384-pad SPectroCHIP (Agena Bioscience, Inc.) using a MassARRAY Nanodispenser RS 1000 (Agena Bioscience, Inc.). .. The spotted chips were placed in the MassARRAY Compact System (Agena Bioscience, Inc.) for detection, followed by spectral acquisition on a MALDI-TOF analyzer (Agena Bioscience, Inc.).

    Article Title: LIN28B Polymorphisms Confer a Higher Postoperative Recurrence Risk in Reproductive-Age Women with Endometrial Polyps
    Article Snippet: .. The PCR products were purified by resin and added into a SpectroCHIP (Agena Bioscience, Sequenom, San Diego, California, USA), then were analyzed by MALDI-TOF techniques. ..

    Article Title: Amplification-free cancer diagnosis based on inhibition of Cas12a activity by site-specific 5mC-modified cfDNA
    Article Snippet: Tissue DNA extracted using the TIANamp Genomic DNA Kit (DP304-03, TIANGEN, China) was treated with the EZ DNA Methylation-GoldTM Kit (D5006, Zymo Research, USA) according to the manufacturer’s instructions. .. Two microliters of the reaction mixture were purified using Agena Resin Clean (8040, Agena Bioscience, USA) and spotted onto SpectroCHIP (10600F, Agena Bioscience, USA) arrays. .. Mass spectra were acquired on the Agena MassARRAY Analyzer 4 using matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) in negative ion mode, and data were processed using MassARRAY Typer software to determine nucleotide-specific modification status.

    Article Title: Amplification-free cancer diagnosis based on inhibition of Cas12a activity by site-specific 5mC-modified cfDNA.
    Article Snippet: Tissue DNA extracted using the TIANamp Genomic DNA Kit (DP304-03, TIANGEN, China) was treated with the EZ DNA Methylation-GoldTM Kit (D5006, Zymo Research, USA) according to the manufacturer’s instructions. .. Two microliters of the reaction mixture were purified using Agena Resin Clean (8040, Agena Bioscience, USA) and spotted onto SpectroCHIP (10600F, Agena Bioscience, USA) arrays. .. Droplets were generated using DG8 cartridges with droplet generation oil in the Bio-Rad Droplet Generator, transferred to a 96-well ddPCR plate, sealed with foil, and amplified in a C1000 Touch Thermal Cycler with the following program: 95 ◦C for 10 min, 40 cycles of 94 ◦C for 30 s and 60 ◦C for 60 s, and 98 ◦C for 10 min. After amplification, droplets were read using the QX200 Droplet Reader and analyzed with QuantaSoft software (Bio-Rad, USA).

    Mass Spectrometry:

    Article Title: Testing calpain inhibition in tumor endothelial cells: novel targetable biomarkers against glioblastoma malignancy.
    Article Snippet: PCR, SAP, and IPLEX reactions were conducted as described in the manufacturer’s protocol (Agena Bioscience, San Diego, CA, USA). .. Samples were transferred to a SpectroCHIP (Agena Bioscience, San Diego, CA, USA) and analyzed by mass spectrometry. .. The spectral profiles frontiersin.org generated by MALDI-TOF mass spectrometry were evaluated using Typer v.4.0 software (Agena Bioscience, San Diego, CA, USA).

    Article Title: Lymphocyte-Rich Spindle Cell Thymoma
    Article Snippet: A study of 80 cases of spindle cell thymoma in which the spindle cell component was overshadowed by massive numbers of stromal lymphocytes is presented.. The patients were 38 women and 42 men, aged 8 to 81 years (mean= 54 y).. All tumors presented as an anterior mediastinal mass; 5 patients had myasthenia gravis and one had Good syndrome.

    Software:

    Article Title: A MALDI-TOF mass spectrometry-based method for detection of copy number variations in BRCA1 and BRCA2 genes
    Article Snippet: Briefly, equal copies of gDNA and competitors were inputted into one tube and PCR amplification was performed as followings: 30°C for 10 min followed by 96°C for 2 min, then 5 cycles of 96°C 45 s, 65°C 30 s and 72°C 1 min, 40 cycles of 96°C 45 s, 60°C 30 s and 72°C 1 min, with a final step of 72°C 5 min. PCR products were then treated with shrimp alkaline phosphatase, followed by a single-base extension which was performed as followings: 97°C 45 s, 97°C 5 s plus 5 repetitions of 52°C 5 s and 80°C 5 s for 40 cycles, and then 72°C 3 min. After extension, the products were desalted and spotted onto a SpectroCHIP (Agena Bioscience) using the MassARRAY Chip Prep Module (Agena Bioscience). .. SpectroCHIPs were scanned using the MassARRAYTM Analyzer 4 (Agena Bioscience) and the spectra were processed using the TYPERTM (Agena Bioscience) software to get the peak intensity for each allele. ..



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